ml si3 Search Results


ml-si3  (MuseChem Chemicals)
N/A
ML-SI3 is a potent TRPML channel inhibitor with IC50s of 4.7 µM and 1.7 µM for TRPML1 and TRPML2, respectively.
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(rel)-ml-si3  (MedChemExpress)
N/A
(rel)-ML-SI3 is one of the active ingredients of ML-SI3 (HY-139426) (another component is (cis)-ML-SI3) that targets three isoforms of TRPML. (rel)-ML-SI3 is an inhibitor of TRPML1 and TRPML3 (IC50=3.1 μM/28.5 μM), and a potent activator
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ml-si3  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH ml-si3
Ml Si3, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ml+si3/pm39201515-202-20-25?v=Biozol+Diagnostica+Vertrieb+GmbH
Average 90 stars, based on 1 article reviews
ml-si3 - by Bioz Stars, 2026-08
90/100 stars
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ml-si3  (MedKoo Inc)
90
MedKoo Inc ml-si3
Effect of TRPML1-agonist ML-SA1 and TRPML1-antagonist <t>ML-SI3</t> on autophagy and α-synuclein particles in human dopaminergic neurons. (A) Example images of LUHMES-derived dopaminergic neurons transduced with α-synuclein-T2A-GFP-lentivirus. Six days after transduction neurons were treated for 24 h either with DMSO or 10 μM ML-SA1. Neurons were further stained against human α-synuclein, lysosomal marker Lamp1 and neuronal marker using MAP2. The scale bar represents 20 μm. (B) Close up of (A) . Closed arrowhead marks a α-synuclein-positive/Lamp1-negative particle, open arrowhead marks a α-synuclein-positive and Lamp1-positive particle, asterisk marks a α-synuclein-negative/Lamp1-positive lysosome. (C) Percentage of Lamp1-positive synuclein particles per neuron. Results of 30 cells per condition. Unpaired t -test depicted with respect to DMSO ( p < 0.001). (D) Representative immunoblot of lysates from transduced LUHMES-derived neurons on day 9, treated with DMSO, ML-SA1 10 μM, or ML-SI3 10 μM for 2 h. Full blot shown in . (E) Quantification of the LC3II band relative to LC3I band in n = 3 independent experiments as in (D) . One-way ANOVA showed no significance ( p = 0.8). (F) Representative immunoblot of lysates from transduced LUHMES-derived neurons on day 9, treated with DMSO, ML-SA1 10 μM or ML-SI3 10 μM for 24 h. Full blot shown in . (G) Quantification of the p62 band relative to beta-Tubulin in n = 3 independent experiments as in (F) . One-way ANOVA showed significance ( p < 0.01). Dunnet’s multiple comparisons test is depicted with respect to DMSO. (H) Quantification of Synuclein load relative to beta-Tubulin in n = 3 independent experiments as in (F) . One-way ANOVA showed no significance ( p = 0.2). ** p < 0.01, *** p < 0.001, non-significant differences are depicted as n.s.
Ml Si3, supplied by MedKoo Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ml+si3/pmc09296810-67-0-1?v=MedKoo+Inc
Average 90 stars, based on 1 article reviews
ml-si3 - by Bioz Stars, 2026-08
90/100 stars
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si 3 ml−1  (BIOTAGE)
90
BIOTAGE si 3 ml−1
Effect of TRPML1-agonist ML-SA1 and TRPML1-antagonist <t>ML-SI3</t> on autophagy and α-synuclein particles in human dopaminergic neurons. (A) Example images of LUHMES-derived dopaminergic neurons transduced with α-synuclein-T2A-GFP-lentivirus. Six days after transduction neurons were treated for 24 h either with DMSO or 10 μM ML-SA1. Neurons were further stained against human α-synuclein, lysosomal marker Lamp1 and neuronal marker using MAP2. The scale bar represents 20 μm. (B) Close up of (A) . Closed arrowhead marks a α-synuclein-positive/Lamp1-negative particle, open arrowhead marks a α-synuclein-positive and Lamp1-positive particle, asterisk marks a α-synuclein-negative/Lamp1-positive lysosome. (C) Percentage of Lamp1-positive synuclein particles per neuron. Results of 30 cells per condition. Unpaired t -test depicted with respect to DMSO ( p < 0.001). (D) Representative immunoblot of lysates from transduced LUHMES-derived neurons on day 9, treated with DMSO, ML-SA1 10 μM, or ML-SI3 10 μM for 2 h. Full blot shown in . (E) Quantification of the LC3II band relative to LC3I band in n = 3 independent experiments as in (D) . One-way ANOVA showed no significance ( p = 0.8). (F) Representative immunoblot of lysates from transduced LUHMES-derived neurons on day 9, treated with DMSO, ML-SA1 10 μM or ML-SI3 10 μM for 24 h. Full blot shown in . (G) Quantification of the p62 band relative to beta-Tubulin in n = 3 independent experiments as in (F) . One-way ANOVA showed significance ( p < 0.01). Dunnet’s multiple comparisons test is depicted with respect to DMSO. (H) Quantification of Synuclein load relative to beta-Tubulin in n = 3 independent experiments as in (F) . One-way ANOVA showed no significance ( p = 0.2). ** p < 0.01, *** p < 0.001, non-significant differences are depicted as n.s.
Si 3 Ml−1, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ml+si3/10__1007_slash_s11104___015___2771___3-96-28-37?v=BIOTAGE
Average 90 stars, based on 1 article reviews
si 3 ml−1 - by Bioz Stars, 2026-08
90/100 stars
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(1s,2s)-ml-si3  (TargetMol)
N/A
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(1s,2s)-ml-si3  (MuseChem Chemicals)
N/A
(1S,2S)-ML-SI3(CAT: I040953) is the trans-isomer of ML-SI3 and acts as a selective inhibitor of TRPML channels. The (-)-isomer is a potent inhibitor of TRPML1 and TRPML2, with IC50 values of 1.6 μM and 2.3 μM,
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ml-si3  (Aladdin Scientific Corporation)
N/A
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ml-si3  (MedChemExpress)
N/A
ML-SI3 is a mixture of cis/trans ML-SI3 (HY-139426A), which is a TRPML1/2 channel inhibitor with IC50s of 4.7 μM and 1.7 μM, respectively. ML-SI3 also inhibits lysosomal calcium efflux and blocks downstream TRPML1-mediated autophagy. The
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(1s,2s)-ml-si3  (MedChemExpress)
N/A
(1S,2S)-ML-SI3 is a trans-isomer of ML-SI3 that targets all three isoforms of TRPML. (1S,2S)-ML-SI3 is an activator of TRPML2 and TRPML3 (EC50=2.7 μM/10.8 μM) and a potent inhibitor of TRPML1 (IC50=5.9 μM).
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(±)-trans-ml-si3  (MuseChem Chemicals)
N/A
(±)-trans-ML-SI3 is an inhibitor of TRPML1 and TRPML3 (IC50 values of 3.1 and 28.5 µM, respectively) and an activator of TRPML2 (EC50 value of 3.3 µM. KEYWORDS: (±)-trans-ML-SI3 | Supplier | TRPML1/3 inhibitor;TRPML2 activator |
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(1r,2r)-ml-si3  (Aladdin Scientific Corporation)
N/A
(1R,2R)-ML-SI3 is a potent inhibitor of both TRPML1 and TRPML2 ( IC 50 values of 1.6 and 2.3 μM) and a weak inhibitor ( IC 50 12.5 μM) of TRPML3Form:SolidIC50& Target:TRPML1 1.6 μM (IC 50
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Image Search Results


Effect of TRPML1-agonist ML-SA1 and TRPML1-antagonist ML-SI3 on autophagy and α-synuclein particles in human dopaminergic neurons. (A) Example images of LUHMES-derived dopaminergic neurons transduced with α-synuclein-T2A-GFP-lentivirus. Six days after transduction neurons were treated for 24 h either with DMSO or 10 μM ML-SA1. Neurons were further stained against human α-synuclein, lysosomal marker Lamp1 and neuronal marker using MAP2. The scale bar represents 20 μm. (B) Close up of (A) . Closed arrowhead marks a α-synuclein-positive/Lamp1-negative particle, open arrowhead marks a α-synuclein-positive and Lamp1-positive particle, asterisk marks a α-synuclein-negative/Lamp1-positive lysosome. (C) Percentage of Lamp1-positive synuclein particles per neuron. Results of 30 cells per condition. Unpaired t -test depicted with respect to DMSO ( p < 0.001). (D) Representative immunoblot of lysates from transduced LUHMES-derived neurons on day 9, treated with DMSO, ML-SA1 10 μM, or ML-SI3 10 μM for 2 h. Full blot shown in . (E) Quantification of the LC3II band relative to LC3I band in n = 3 independent experiments as in (D) . One-way ANOVA showed no significance ( p = 0.8). (F) Representative immunoblot of lysates from transduced LUHMES-derived neurons on day 9, treated with DMSO, ML-SA1 10 μM or ML-SI3 10 μM for 24 h. Full blot shown in . (G) Quantification of the p62 band relative to beta-Tubulin in n = 3 independent experiments as in (F) . One-way ANOVA showed significance ( p < 0.01). Dunnet’s multiple comparisons test is depicted with respect to DMSO. (H) Quantification of Synuclein load relative to beta-Tubulin in n = 3 independent experiments as in (F) . One-way ANOVA showed no significance ( p = 0.2). ** p < 0.01, *** p < 0.001, non-significant differences are depicted as n.s.

Journal: Frontiers in Cellular Neuroscience

Article Title: Activated Endolysosomal Cation Channel TRPML1 Facilitates Maturation of α-Synuclein-Containing Autophagosomes

doi: 10.3389/fncel.2022.861202

Figure Lengend Snippet: Effect of TRPML1-agonist ML-SA1 and TRPML1-antagonist ML-SI3 on autophagy and α-synuclein particles in human dopaminergic neurons. (A) Example images of LUHMES-derived dopaminergic neurons transduced with α-synuclein-T2A-GFP-lentivirus. Six days after transduction neurons were treated for 24 h either with DMSO or 10 μM ML-SA1. Neurons were further stained against human α-synuclein, lysosomal marker Lamp1 and neuronal marker using MAP2. The scale bar represents 20 μm. (B) Close up of (A) . Closed arrowhead marks a α-synuclein-positive/Lamp1-negative particle, open arrowhead marks a α-synuclein-positive and Lamp1-positive particle, asterisk marks a α-synuclein-negative/Lamp1-positive lysosome. (C) Percentage of Lamp1-positive synuclein particles per neuron. Results of 30 cells per condition. Unpaired t -test depicted with respect to DMSO ( p < 0.001). (D) Representative immunoblot of lysates from transduced LUHMES-derived neurons on day 9, treated with DMSO, ML-SA1 10 μM, or ML-SI3 10 μM for 2 h. Full blot shown in . (E) Quantification of the LC3II band relative to LC3I band in n = 3 independent experiments as in (D) . One-way ANOVA showed no significance ( p = 0.8). (F) Representative immunoblot of lysates from transduced LUHMES-derived neurons on day 9, treated with DMSO, ML-SA1 10 μM or ML-SI3 10 μM for 24 h. Full blot shown in . (G) Quantification of the p62 band relative to beta-Tubulin in n = 3 independent experiments as in (F) . One-way ANOVA showed significance ( p < 0.01). Dunnet’s multiple comparisons test is depicted with respect to DMSO. (H) Quantification of Synuclein load relative to beta-Tubulin in n = 3 independent experiments as in (F) . One-way ANOVA showed no significance ( p = 0.2). ** p < 0.01, *** p < 0.001, non-significant differences are depicted as n.s.

Article Snippet: ML-SI3 (MedKoo Biosciences, Inc., Morrisville, USA) was dissolved in DMSO to a 25 mM stock solution using a final concentration on neurons as depicted.

Techniques: Derivative Assay, Transduction, Staining, Marker, Western Blot

Effect of TRPML1-agonist ML-SA1 and TRPML1-antagonist ML-SI3 on α-synuclein particles in iPSC-derived mature neurons. (A) Example images of NPC-derived neurons transduced with α-synuclein-T2A-GFP using lentivirus. Twenty-one days after transduction, cells were treated for 24 h either with 20 μM ML-SA1 or DMSO. Cells were further stained against human α-synuclein, lysosomal marker Lamp1 and neuronal marker MAP2. The scale bar represents 20 μm. (B) Close up of (A) . Closed arrowhead marks a α-synuclein-positive/Lamp1-negative particle, open arrowhead marks a α-synuclein-positive and Lamp1-positive particle, asterisk marks a α-synuclein-negative/Lamp1-positive lysosome. (C) Percentage of Lamp1-positive synuclein particles per cell. Results of at least 30 neurons per condition. Unpaired t -test depicted with respect to DMSO ( p = 0.01). (D) Representative immunoblot of lysates from transduced neurons on day 21, treated with DMSO, ML-SA1 10 μM, or ML-SI3 10 μM and additionally DMSO or 100 nM BafA1 for 2 h. Full blot shown in . (E) Quantification of the Synuclein-T2A-dimer band relative to total synuclein load in n = 3 independent experiments as in (D) . One-way ANOVA showed significance ( p = 0.03). Results of Dunnet’s multiple comparisons test are depicted with respect to DMSO ( p = 0.03). (F) Quantification of Synuclein load relative to beta-Tubulin in n = 3 independent experiments as in (D) . Two-way-ANOVA showed significant interaction ( p = 0.03), Sidak’s multiple comparisons test is depicted with respect to DMSO. * p < 0.05, ** p < 0.01, non-significant differences are depicted as n.s.

Journal: Frontiers in Cellular Neuroscience

Article Title: Activated Endolysosomal Cation Channel TRPML1 Facilitates Maturation of α-Synuclein-Containing Autophagosomes

doi: 10.3389/fncel.2022.861202

Figure Lengend Snippet: Effect of TRPML1-agonist ML-SA1 and TRPML1-antagonist ML-SI3 on α-synuclein particles in iPSC-derived mature neurons. (A) Example images of NPC-derived neurons transduced with α-synuclein-T2A-GFP using lentivirus. Twenty-one days after transduction, cells were treated for 24 h either with 20 μM ML-SA1 or DMSO. Cells were further stained against human α-synuclein, lysosomal marker Lamp1 and neuronal marker MAP2. The scale bar represents 20 μm. (B) Close up of (A) . Closed arrowhead marks a α-synuclein-positive/Lamp1-negative particle, open arrowhead marks a α-synuclein-positive and Lamp1-positive particle, asterisk marks a α-synuclein-negative/Lamp1-positive lysosome. (C) Percentage of Lamp1-positive synuclein particles per cell. Results of at least 30 neurons per condition. Unpaired t -test depicted with respect to DMSO ( p = 0.01). (D) Representative immunoblot of lysates from transduced neurons on day 21, treated with DMSO, ML-SA1 10 μM, or ML-SI3 10 μM and additionally DMSO or 100 nM BafA1 for 2 h. Full blot shown in . (E) Quantification of the Synuclein-T2A-dimer band relative to total synuclein load in n = 3 independent experiments as in (D) . One-way ANOVA showed significance ( p = 0.03). Results of Dunnet’s multiple comparisons test are depicted with respect to DMSO ( p = 0.03). (F) Quantification of Synuclein load relative to beta-Tubulin in n = 3 independent experiments as in (D) . Two-way-ANOVA showed significant interaction ( p = 0.03), Sidak’s multiple comparisons test is depicted with respect to DMSO. * p < 0.05, ** p < 0.01, non-significant differences are depicted as n.s.

Article Snippet: ML-SI3 (MedKoo Biosciences, Inc., Morrisville, USA) was dissolved in DMSO to a 25 mM stock solution using a final concentration on neurons as depicted.

Techniques: Derivative Assay, Transduction, Staining, Marker, Western Blot